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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Exp Eye Res. 2015 Feb 24;135:127–133. doi: 10.1016/j.exer.2015.02.018

Figure 1. ASCs exhibited a time and dose dependent response to aqueous humor.

Figure 1

ASCs were cultured in full media (serum supplemented DMEM) in the presence of 5, 10, or 20% AH. (A) Cells were stained for alkaline phosphatase (ALP) activity (red) and with Alcian blue (blue). Some ALP activity was observed in untreated cells (Control), but cells treated with AH exhibit dramatic increase in ALP activity, exemplified by cells cultured for 3 weeks in the presence of 5% AH (3 week - 5% AH). There was apparently less effect of AH on Alcian blue staining. (B) At 2 weeks, ALP activity was increased in a dose dependent manner, with 10% and 20% eliciting a significant response when compared to control cells. (C) At 3 weeks, all doses resulted in a significant increase in ALP activity compared to control. All data were normalized to the response of 3 week treatment with 20% AH. Data are mean ± SEM (n = 3 donors). * p < 0.05; ** p < 0.01; *** p < 0.001. Scale bar 50 μm.