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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Exp Eye Res. 2015 Feb 24;135:127–133. doi: 10.1016/j.exer.2015.02.018

Figure 3. The effect of aqueous humor on the osteogenic potential of ASCs was dependent on thermally labile and charcoal-adsorbable components.

Figure 3

ASCs were cultured for 3 weeks in full media (serum supplemented DMEM) in the presence of 5% AH. The AH was either untreated, incubated with dextran-coated charcoal (DCC), or heat treated. (A) Both DCC (5% AH/DCC) and HT (5% AH/HT) treatments reduced ALP activity when compared to untreated AH (5% AH), with HT reducing activity to the level seen in untreated cells (Control). (B) Both DCC and HT significantly reduced the ALP induction caused by AH. The effect of DCC was partial, and was still significantly elevated compared to control cells, while HT resulted in complete loss of AH osteogenic activity. All data normalized to the response of 5% AH case. Data are mean ± SEM (n = 3 donors). * p < 0.05; ** p < 0.01; *** p < 0.001. Scale bar 50 μm.