Fig. 12. AT2R-eGFP cells surrounding the PVN express Gad1.
(a) Low magnification image through the PVN (−1.06 mm caudal to bregma) of an AT2R-eGFP reporter mouse co-labeled for AT2R-eGFP (green) and Gad1 mRNA (red). (b-d) Projection images of neurons surrounding the PVN depicting (b) Gad1 mRNA (red), (c) AT2R-eGFP (green) and (d) the merged image. 3v = third ventricle. Scale bars = (a) 100 μm and (b-d) 50 μm.