Fig 6. Effect of photofading during image acquisition on fits to FRAP curves for normal diffusion versus anomalous diffusion.

FRAP data with photofading (•) were simulated using f _dADaMf(t) (Eq 42) assuming either a photofading rate of κ = 0.04 s⁻¹ (A,B) or κ = 0.01 s⁻¹ (C,D) under the conditions D = 1μm²/s, F ₀ = 0.2, M _f = 0.8, r _n = 0.2 μm, and r _e = 1 μm. The simulated curve was next fitted by f _NDaMf(t) (Eq 37) (A,C) or f _NDaMf(t) (Eq 37) (B,D). The resulting best fitting parameters were D = 2.1μm²/s and M _f = 0.83 in (A), $\frac{1}{4}\Gamma =9.33$ and M _f = 0.83 in (B), D = 2.7μm²/s and M _f = 0.95 in (C), and $\frac{1}{4}\Gamma =5.32$ and M _f = 0.94 in (D).