Table 2. Odorant receptor (OR) mRNA expression in antennal and body tissue of female and male Epiphyas postvittana as determined by quantitative RT-PCR and OR transcript counts in antennal tissue as determined by RNAseq.
| qPCR data (fold change to reference genes) | RNASeq Data (FPKM) | |||||
|---|---|---|---|---|---|---|
| Gene | female antennae | female body | male antennae | male body | female antennae | male antennae |
| EposOR1 | 0.03263 | 0.00024 | 0.07130 | BLD | 191 | 104 |
| EposOrco | 2.62100 | BLD | 3.55188 | BLD | 2676 | 1397 |
| EposOR3 | 0.00768 | BLD | 0.00663 | BLD | 35 | 30 |
| EposOR4 | 0.03408 | BLD | 0.03337 | 0.00011 | 72 | 53 |
| EposOR5 | 0.01009 | 0.00002 | 0.00138 | BLD | 319 | 37 |
| EposOR6 | 0.00073 | BLD | 0.04183 | 0.00600 | 0 | 12 |
| EposOR7 | 0.00315 | 0.00010 | 0.01561 | 0.00138 | 0 | 90 |
| EposOR8 | 0.00053 | BLD | 0.00006 | 0.00002 | 0 | 0 |
| EposOR9 | 0.00145 | BLD | 0.00127 | BLD | 18 | 6 |
| EposOR10 | 0.00791 | 0.00007 | 0.00624 | 0.00115 | 37 | 9 |
| EposOR11 | BLD | BLD | BLD | BLD | 0 | 0 |
| EposOR12 | 0.00143 | BLD | 0.00192 | 0.00001 | 74 | 74 |
| EposOR13 | 0.00028 | 0.00004 | 0.00071 | 0.00059 | 0 | 0 |
| EposOR14 | 0.01350 | 0.00003 | 0.00650 | 0.00028 | 35 | 12 |
| EposOR15 | 0.03509 | 0.00502 | 0.02606 | 0.02465 | 73 | 34 |
| EposOR16 | 0.00265 | BLD | 0.00726 | 0.00023 | 3 | 1 |
| EposOR17 | 0.00560 | 0.00493 | 0.01428 | 0.00325 | 2 | 1 |
| EposOR18 | 0.03153 | 0.00029 | 0.02091 | BLD | 63 | 28 |
| EposOR19 | 0.07996 | 0.00007 | 0.03561 | BLD | 288 | 57 |
| EposOR20 | 0.01524 | 0.00139 | 0.01714 | 0.00381 | 74 | 23 |
| EposOR21 | 0.00729 | 0.00002 | 0.00099 | 0.00003 | 66 | 6 |
| EposOR22 | 0.07837 | 0.00007 | 0.02709 | BLD | 108 | 43 |
| EposOR23 | 0.00058 | 0.00025 | 0.00145 | 0.00108 | 1 | 1 |
| EposOR24 | 0.01978 | BLD | 0.02527 | BLD | 20 | 9 |
| EposOR25 | 0.03330 | 0.00034 | 0.03440 | 0.00260 | 46 | 15 |
| EposOR26 | 0.05425 | 0.01337 | 0.11274 | 0.01719 | 21 | 15 |
| EposOR27 | 0.05022 | BLD | 0.04528 | 0.00128 | 104 | 46 |
| EposOR28 | 0.01670 | BLD | 0.00903 | 0.00009 | 22 | 20 |
| EposOR29 | 0.04849 | 0.00020 | 0.02772 | 0.00027 | 36 | 18 |
| EposOR30 | 0.02020 | 0.00398 | 0.69629 | 0.02740 | 0 | 536 |
| EposOR31 | 0.00616 | BLD | 0.00003 | BLD | 189 | 0 |
| EposOR32 | 0.04552 | BLD | 0.03881 | BLD | 18 | 6 |
| EposOR33 | 0.00640 | BLD | 0.00047 | 0.00004 | 60 | 0 |
| EposOR34 | 0.01131 | 0.00043 | 0.32708 | 0.00233 | 1 | 779 |
| EposOR35 | 0.00408 | BLD | 0.00543 | BLD | 26 | 14 |
| EposOR36 | 0.02528 | BLD | 0.00014 | 0.00004 | 57 | 0 |
| EposOR37 | 0.00393 | 0.00008 | 0.01092 | 0.00008 | 20 | 17 |
| EposOR38 | 0.04631 | 0.00006 | 0.11024 | 0.00197 | 50 | 19 |
| EposOR39 | 0.09887 | BLD | 0.19689 | BLD | 50 | 24 |
| EposOR40 | 0.32466 | 0.00007 | 0.00507 | 0.00135 | 383 | 1 |
| EposOR41 | 0.00446 | 0.00019 | 0.00911 | BLD | 29 | 19 |
| EposOR42 | 0.05713 | BLD | 0.09239 | BLD | 70 | 47 |
| EposOR43 | 0.09436 | 0.00068 | 0.04906 | 0.00181 | 21 | 17 |
| EposOR44 | 0.00199 | BLD | 0.00317 | BLD | 44 | 19 |
| EposOR45 | 0.01907 | 0.00015 | 0.02347 | 0.00011 | 147 | 75 |
| EposOR46 | 0.00932 | 0.00015 | 0.01168 | 0.00079 | 28 | 16 |
| EposOR47 | 0.08845 | 0.00096 | 0.07746 | 0.00430 | 52 | 31 |
| EposOR48 | 0.06465 | 0.00007 | 0.07733 | 0.00034 | 102 | 75 |
| EposOR49 | 0.00271 | 0.00022 | 0.00522 | 0.00008 | 6 | 5 |
| EposOR50 | 0.00054 | 0.00008 | 0.00144 | 0.00010 | 3 | 1 |
| EposOR51 | 0.05788 | 0.00038 | 0.06611 | 0.00031 | 163 | 84 |
| EposOR52 | 0.00986 | BLD | 0.00955 | BLD | 24 | 9 |
| EposOR53 | 0.01144 | 0.00003 | 0.01077 | 0.00011 | 128 | 92 |
| EposOR54 | 0.02487 | 0.00003 | 0.02158 | 0.00011 | 47 | 23 |
| EposOR55 | 0.00098 | 0.00001 | 0.00569 | 0.00026 | 37 | 28 |
| EposOR56 | 0.00100 | BLD | 0.00071 | BLD | 10 | 4 |
| EposOR57 | 0.00114 | BLD | 0.00610 | 0.00001 | 8 | 3 |
| EposOR58 | 0.00405 | 0.00001 | 0.00339 | 0.00003 | 81 | 40 |
| EposOR59 | 0.01240 | 0.00061 | 0.00962 | 0.00044 | 11 | 3 |
| EposOR60 | 0.00464 | 0.00010 | 0.01035 | BLD | 38 | 32 |
| EposOR61 | 0.01409 | 0.00006 | 0.01719 | BLD | 160 | 50 |
| EposOR62 | 0.00589 | BLD | 0.00451 | BLD | 30 | 16 |
| EposOR63 | 0.00017 | BLD | 0.00011 | BLD | 7 | 3 |
| EposOR64 | 0.05393 | BLD | 0.01088 | BLD | 227 | 56 |
| EposOR65 | 0.01115 | 0.00005 | 0.00184 | 0.00004 | 108 | 17 |
| EposOR66 | 0.00506 | 0.00020 | 0.00296 | BLD | 14 | 4 |
| EposOR67 | 0.02124 | 0.00001 | 0.01068 | 0.00007 | 128 | 43 |
| EposOR68 | 0.02752 | 0.00027 | 0.02945 | BLD | 55 | 32 |
| EposOR69 | BLD | BLD | BLD | BLD | 0 | 0 |
| EposOR70 | BLD | BLD | BLD | BLD | 1 | 0 |
Quantitative RT-PCR data represent the mean fold change to reference genes from three biological replicates. RNAseq data represent transcript counts from a single transcriptome made from male or female antennal mRNA. BLD = Below Limit of Detection.