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. 2015 May 27;10(5):e0123971. doi: 10.1371/journal.pone.0123971

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© 2015 Lin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A and B) EPCs were pretreated with anti-SR-A, anti-SR-B1, or LOX-1 blocking antibodies for 1 hour prior to oxLDL treatment. The effect of oxLDL on EPC neovascularization was analyzed using an in vitro angiogenesis assay. (C) EPCs were treated for 12 hours with 0–50 μg/mL oxLDL, and membrane LOX-1, SR-A, and SR-B1 were analyzed by Western blot. α-tubulin protein levels were used as a loading control. (D) The graph shows the quantification of LOX-1, SR-A, and SR-B1 density in oxLDL-treated EPCs. Data are expressed as the mean ± SEM of three experiments. *p < 0.05 was compared to the control group in the same time treatment and considered significant.