Table 1. Real-time PCR primers and probes.
Primer | Primer sequence |
Line-1 F | 5′TTATTAGGGAGTGTTAGATAGTGGG3′ |
Line-1 R | 5′CCTCTAAACCAAATATAAAAT ATAATCTC3′; |
ALU-C4 F | 5’GGTTAGGTATAGTGGTTTATATTTGTAATTTTAGTA3’ |
ALU-C4 R | 5’ATTAACTAAACTAATCTTAAACTCCTAACCTCA3’ |
Probe | Probe sequence |
Line-1 met | VIC–TACTTCGACTCGCGCACGATA-MBG3' |
Line-1 unmet | 6FAM–CCTACTTCAACTCACACA-MBG3' |
ALU-C4 | 6FAM-CCTACCTTAACCTCCC-MGB3' |