TABLE 1.
Serological results of ELISAs for the presence of FeLV p27 antigens and antibodies against FeLV p45 and FeLV whole virus (FL-74) in free-ranging, captive nonvaccinated, and captive vaccinated cheetahs
| ELISAa | Free-ranging |
Captive nonvaccinated |
Captive vaccinated |
|||
|---|---|---|---|---|---|---|
| No. (%) of animals | No. (%) of samples | No. (%) of animals | No. (%) of samples | No. (%) of animals | No. (%) of samples | |
| P27 | ||||||
| >20% (positive) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| ≥4% to ≤20% | 6 (7) | 6 (6) | 3 (11) | 3 (8) | 2 (6) | 2 (6) |
| <4% (negative) | 82 (93) | 92 (94) | 25 (89) | 36 (92) | 33 (94) | 33 (94) |
| Total tested | 88 | 98 | 26 | 39 | 35 | 35 |
| Sum | 88 (100) | 98 (100) | 28 (100)b,c | 39 (100) | 35 (100) | 35 (100) |
| P45 | ||||||
| >20% (positive) | 1 (1) | 1 (1) | 3 (11) | 6 (20) | 25 (71) | 25 (71) |
| ≤20% (negative) | 69 (99) | 74 (99) | 24 (89) | 24 (80) | 10 (29) | 10 (29) |
| Total tested | 70 | 75 | 26 | 30 | 35 | 35 |
| Sum | 70 (100) | 75 (100) | 27 (100)b,c | 30 (100) | 35 (100) | 35 (100) |
| FL-74 | ||||||
| >22% (positive) | 17 (19) | 21 (21) | 7 (25) | 9 (23) | 30 (86) | 30 (86) |
| ≤22% (negative) | 72 (81) | 77 (79) | 21 (75) | 30 (77) | 5 (14) | 5 (14) |
| Total tested | 88 | 98 | 26 | 39 | 35 | 35 |
| Sum | 89 (100)b | 98 (100) | 28 (100)b | 39 (100) | 35 (100) | 35 (100) |
Samples with OD values of ≥20% (P27 and P45) or ≥22% (FL-74) were considered positive; samples with OD values of ≥4% and ≤20% were considered questionable and samples with OD values of <4% were considered negative for p27 antigens.
The sum of the animals is greater than the total number of animals because animals captured and tested repeatedly were classified in different OD levels (see the text).
The sum of the animals is greater than the total number of animals because animals tested once with plasma and once with serum were classified in different OD levels (see the text).