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(A) Sequence, secondary structure and 3D structure of 1AFX. (B) Base-stacking energies between adjacent two nucleotides in 1AFX calculated by 3dRNAscore. ‘1–2′ means base-stacking energy between the first and the second nucleotide, ‘2–3′ means the second to the third … and so on. The lower the energy, the better the base-stacking. (C) Base-pairing energies between each possible base-pair in 1AFX calculated by 3dRNAscore. The lower the energy, the better the base-pairing.
