Table 3. Types of variations in protein coding features.
| Feature | Synonymous codon changesa | Conservative residue changesa | Non-conservative residue changesa | Variants no aa changesb |
|---|---|---|---|---|
| AmpR/bla(M) | 73% | 17% | 10% | 39% |
| CmR | 84% | 16% | 0% | 81% |
| HygR | 87% | 1% | 12% | 57% |
| KanR | 54% | 6% | 40% | 32% |
| NeoR/KanR | 62% | 14% | 24% | 65% |
| PuroR | 94% | 1% | 5% | 73% |
| lacZ-αc | 93% | 7% | 0% | 95% |
| MBP | 27% | 2% | 71% | 60% |
| Total no. | 641 | 54 | 156 | 134 |
| Total Mean | 75% | 7% | 18% | 71% |
aPercentage of all bp changes including mismatches and indels but excluding border differences.
bPercentage of variants that produce no changes in the translated protein.
cExcluding the multiple cloning sites.