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. 2015 Apr 27;43(10):5158–5170. doi: 10.1093/nar/gkv287

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Molecular characterization of small-molecule-sensing regazyme. (A) Sequence and structure of the regazyme theoHHAzRAJ12. A small molecule (Theo) binds to the regazyme to reconstitute the active conformation of the ribozyme and then produce the cleavage. An arrow marks the cleavage site, between the transducer module and the ribozyme core. The seed of the riboregulator is paired in the uncleaved state. (B) Time-dependent electrophoretic analysis of cellular RNA extracts taken at different time points; gel shown for 4 mM Theo. Quantification of dynamic RNA processing for different concentrations of the signal molecule (Theo). Data fitted with a generalized exponential decay model with production, where the temporal factor is (1 − exp(−λt))^m, with m ≈ 1. Error bars represent standard deviations over replicates.