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. 2015 Apr 27;43(10):4868–4880. doi: 10.1093/nar/gkv388

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Kinetic analysis of LSD1/CoREST demethylase on nucleosome substrates. (A) Schematic of Amplex Ultrared based fluorescence LSD1/CoREST demethylase assay. The hydrogen peroxide generated in the histone demethylase assay drives the horseradish peroxidase catalyzed conversion of Amplex UltraRed to the highly fluorescent resorufin molecule. (B) Michaelis–Menten analysis of LSD1/CoREST demethylase activity on nucleosomes with symmetrical extranucleosomal DNA. Kinetic data for 147 bp (1N1), 157 bp (6N6), 165 bp (10N10) and 185 bp (20N20) are shown in yellow, blue, green and red, respectively. Each data point was measured at least three times.