Table 2. Dissociation constants for LSD1 complex binding to nucleosomes with different length extranucleosomal DNA at 50 mM NaCl.

Label position	DNA length	Histones	DNA format	Kd (nM)	Relative to 147 bp
H3 K27C	147	WT	1 + 145 + 1	1641 ± 211	1.0
	151	WT	3 + 145 + 3	716 ± 100	2.3
	155	WT	5 + 145 + 5	419 ± 23	3.9
	157	WT	6 + 145 + 6	470 ± 64	3.5
	159	WT	7 + 145 + 7	236 ± 40	7.0
	161	WT	8 + 145 + 8	232 ± 29	7.1
	163	WT	9 + 145 + 9	237 ± 22	6.9
	165	WT	10 + 145 + 10	205 ± 26	8.0
	169	WT	12 + 145 + 12	282 ± 68	5.8
	177	WT	16 + 145 + 16	214 ± 35	7.7
	181	WT	18 + 145 + 18	100 ± 27	16.2
	185	WT	20 + 145 + 20	135 ± 27	12.1
	207	WT	31 + 145 + 31	263 ± 27	6.2
	151L	WT	5 + 145 + 1	718 ± 31	2.3
	161L	WT	15 + 145 + 1	406 ± 52	4.0
	177L	WT	31 + 145 + 1	769 ± 174	2.1
	177	Acidic patch mutation	16 + 145 + 16	98 ± 27	16.7
H3 A21C	147	WT	1 + 145 + 1	81.0 ± 9.0	1.0
	157	WT	6 + 145 + 6	17.6 ± 1.9	4.6
	181	WT	18 + 145 + 18	3.8 ± 1.1	21.4
	207	WT	31 + 145 + 31	3.0 ± 1.3	27.2