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. 2015 Apr 27;43(10):4909–4922. doi: 10.1093/nar/gkv405

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — CENP-B binds to the CENP-A and H3.1 nucleosomes. (A) Schematic representation of CENP-B DBD binding to nucleosomes. (B) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1^CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by non-denaturing 6% polyacrylamide gel electrophoresis with ethidium bromide staining. (C) Protein contents of the H3.1, CENP-A and H3.1^CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.