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. 2015 Apr 27;43(10):4909–4922. doi: 10.1093/nar/gkv405

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — CENP-B binds to the CENP-A nucleosome more stably than the H3.1 nucleosome. (A) Schematic representation of the competitive CENP-B retention assay. (B) The H3.1 or CENP-A nucleosomes (containing 100 ng DNA) complexed with the CENP-B DBD were incubated in the presence of a naked 166 base-pair DNA containing the CENP-B box sequence. Lanes 1–6 and lanes 7–12 indicate the experiments with the H3.1 and CENP-A nucleosomes, respectively. The amounts of naked 166 base-pair DNA are 0 ng (lanes 2 and 8), 25 ng (lanes 3 and 9), 50 ng (lanes 4 and 10), 75 ng (lanes 5 and 11), and 100 ng (lanes 6 and 12). Lanes 1 and 7 indicate control experiments without the CENP-B DBD and the naked 166 base-pair DNA. (C) Graphic representation of the experiments shown in panel (B). The amounts (%) of nucleosomes complexed with the CENP-B DBD were plotted against the amounts of competitor DNA. Averages of four independent experiments are shown with standard deviation values.