Figure 4.

eIF3 is one of the major players in programmed stop codon readthrough. The PBH140 derivatives bearing TIF35 wt and tif35-KLF mutant alleles (generated as described in Figure 2) were grown and processed for stop codon readthrough measurements as described in Materials and Methods, except that besides our standard (‘control’) pTH477 readthrough construct (shown in Figure 1A), five derivatives with variable hexanucleotide sequence immediately following the stop codon were also employed: PBB85 (for SUP45), PBB83 (for ADE1), PBB84 (for PDE2), PBB80 (for BSC4) and PBB82 (for TMV).