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. 2015 Apr 29;43(10):5099–5111. doi: 10.1093/nar/gkv421

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Human eIF3 promotes stop codon readthrough. HeLa cells were transfected with siRNA against the eIF3a and eIF3g subunits (eIF3a^K.D. and eIF3g^K.D.) or non-targeting siRNA (nt) as control. Two days later, the siRNA-treated cells were further transfected with the bicistronic firefly/renilla luciferase readthrough reporters (40). Both luciferases are separated by a stop (UAG) or sense codon (CAG) followed—in both cases—by a highly readthrough permissive sequence (CAAUUA) from TMV (41). The UAG-C readthrough is displayed as % of the signal obtained with the sense codon reporter; standard deviations are given.