Figure 1.

Identification of PPNs and of the recorded PPNs that were immunostained for MOR. (A₁) sacral parasympathetic nucleus (SPN) located in the mediolateral border of the gray matter that was identified under a lower magnification with fluorescent illumination. (A₂) tetramethylrhodamine-dextran (TMR)-labeled PPNs were identified at a higher magnification with fluorescent illumination. (A₃) The neuron shown in (A₂), but viewed with infrared illumination during whole-cell recording. (B₁,C₁,D₁) The recorded neuron shown in (A₂,A₃), filled with biocytin and visualized with FITC-conjugate avidin (green) at various magnifications. (B₂,C₂,D₂) The same section shows TMR immunoreactivity (Alexa 594) and is shown at the same magnification as in (B₁,C₁,D₁), respectively. (B₃,C₃,D₄) Merged images show that the biocytin-filled neuron was the TMR-containing neuron. (D₃) The neuron also shows MOR-Immunoreactive (IR) (Alexa 647). (D₄) Merged image shows that the biocytin-filled neuron was the TMR-containing neuron that exhibited MOR-IR. Scale bars: 7 μm in (B); 20 μm in (C); 100 μm in (D).