FIGURE 10.

Patch clamp recordings from Layer II pyramidal cells show that CRF and DOI reduced action potential firing. In (A) a current clamp recording from a pyramidal cell we show that CRF and subsequently DOI attenuated the excitation produced by a 1 s 80 Hz pulse. In (B) a raster plot of 18 recordings from Layer II cells shows a summary of these data. In (C) like in the VSD recordings the effects of CRF were slowly reversible. In (D) a current clamp recording from a typical pyramidal cell shows that the number of action potentials produced by current injection over a range of current steps was reduced by CRF and DOI. This effect was primarily due to increased accommodation with no prominent change in firing frequency (see text for a more complete description).