FIGURE 2.

Activation of CRFR₁ in PC reduced the activation of pyramidal cell layer (Layer II) and dorsal endopiriform nucleus (DEn) but attenuated the deactivation of interneuronal layer (Layer III). Representative images taken 1 s after the end of stimulation are shown in (A) before (left) and after the application of 100 nM CRF (middle 15 min) and after 20 min wash (right). In (B–D) we show quantification of CRFR₁ effects over the range of stimulation frequencies used to activate the circuit. CRF was most effective on the 60 and 80 Hz stimulations. ^*p < 0.05.