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. 2015 May 28;9:72. doi: 10.3389/fnana.2015.00072

Figure 5.

Figure 5

Immunohistochemistry of mesencephalic neuroepithelial explants under various experimental conditions. Optic tectum explants from HH20 embryos cultured for 24 h in the presence of: (i) DMEM as a negative control (A,D); (ii) Conditioned medium from SCO culture explants (B,E); (iii) Conditioned medium from SCO culture explants plus anti-SCO-spondin antibodies (C,F); (iv) DMEM in the presence of LDL (G,J); (v) Conditioned medium from SCO culture explants in the presence of LDL (H,K); and (vi) The same as (v), but with anti-SCO-Spondin (I,L). The explants were analyzed for the presence of βIII tubulin (A–C, G–I) and BrdU incorporation (D–F, J–L). Panels (A′–L′) show the merger, with the TO-PRO-3 nuclear signal used to counterstain the tissue in red. Scale bar in A is 50 μm, all the images have the same magnification.