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. 2015 May 28;6:516. doi: 10.3389/fmicb.2015.00516

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Copyright © 2015 Lies, Visser, Joshi, Magnan and Bates.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mioC and gidA promoter mutants are elongated. Cells carrying promoter mutations (PmioC, PgidA) in a fis⁺or fis⁻ genetic background were diluted 1:1600 in LB medium at 37°C and assayed for growth and cell division. (A,B) Phase contrast images of cells at mid-exponential phase (OD600 = 0.2). (C,D) Above cultures were assayed for mass doubling time by optical density at 600 nm (light gray bars), cell number doubling time by flow counting in a Coulter particle counter (dark gray bars), and cell length at OD600 = 0.2 by direct microscopic observation (hatched bars, n = 500–600 cells). Values are means of 5 independent experiments ±1 standard deviation (SD).