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. 2015 Apr 29;13(5):2666–2679. doi: 10.3390/md13052666

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effect of PBME on H₂O₂-induced DNA damage in the C2C12 cells. The C2C12 cells were pretreated with 300 μg/mL of PBME for 1 h and then incubated with and without 1 mM of H₂O₂ for 6 h. (A) To detect cellular DNA damage, a comet assay was performed, and representative pictures of the comets were taken using a fluorescence microscope (200× original magnification); (B) The cells were lysed, and equal amounts of cell lysates were then separated on SDS-polyacrylamide gels and transferred to nitrocellulose membranes. The membranes were probed with specific antibodies against p-γH2A.X and actin, as an internal control, and the proteins were visualized using an enhanced chemiluminescence (ECL) detection system. A representative blot from three independent experiments is shown.

Effect of PBME on H₂O₂-induced DNA damage in the C2C12 cells. The C2C12 cells were pretreated with 300 μg/mL of PBME for 1 h and then incubated with and without 1 mM of H₂O₂ for 6 h. (A) To detect cellular DNA damage, a comet assay was performed, and representative pictures of the comets were taken using a fluorescence microscope (200× original magnification); (B) The cells were lysed, and equal amounts of cell lysates were then separated on SDS-polyacrylamide gels and transferred to nitrocellulose membranes. The membranes were probed with specific antibodies against p-γH2A.X and actin, as an internal control, and the proteins were visualized using an enhanced chemiluminescence (ECL) detection system. A representative blot from three independent experiments is shown.