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. 2015 Apr 29;13(5):2666–2679. doi: 10.3390/md13052666

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effects of an inhibitor of HO-1 on PBME-mediated attenuation of ROS formation, apoptosis, and growth inhibition by H₂O₂ in the C2C12 cells. (A) The cells grown under the same conditions as in Figure 5 and assayed for ROS generation; (B) The degree of apoptosis was evaluated using a flow cytometer; (C) The cell viability was estimated with an MTT assay. The results are presented as the mean ± SD values obtained in three independent experiments (* p < 0.05 compared with the control group; ^# p < 0.05 compared with the H₂O₂-treated group; ^$ p < 0.05 compared with the H₂O₂- and PBME-treated group).