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. 2015 May 6;13(5):2813–2833. doi: 10.3390/md13052813

Figure 1.

Figure 1

Tilapia piscidin 4 (TP4) does not exhibit cytotoxic effects on a human fibroblast cell line (Hs68), and actually stimulates proliferation activity. (A–C) Hs68 cells were treated with different doses of TP4 for 48 h, and cell viability was measured by neutral red, LDH, and MTT assays; (D–F) Experimental Hs68 cells were treated with TP4 (6.25 μg/mL), while control cells were untreated. After 48 h, total RNA was isolated and reverse transcribed for use in real-time qPCR analysis. Values with different letters show significant differences (P < 0.05), as determined by ANOVA. The lowercase letters indicate the following: n.s., not significant; * significant (P < 0.05); ** significant (P < 0.001).