Fig. 1. Images of Der p2-EGFP and co-localized with ER-RFP in BEAS-2B cells by confocal laser scanning biological microscopy. Cells were cultured with 5 µg/mL of rDer p2-EGFP or rEGFP alone. No fluorescence could be detected in cells incubated with rEGFP alone (A). Fluorescence from Der p2-EGFP appeared in the cytoplasm after 4-hour (B) and 24-hour (C) incubation. Cells were also co-transduced with CellLight™ ER-RFP. Der p2-EGFP co-localized with ER-RFP after 24-hour incubation (C). A representative image and an actual figure representative of the 3 independent experiments with similar findings are shown. DIC, differential interference contrast; EGFP, enhanced green fluorescent protein; ER-RFP, endoplasmic reticulum-red fluorescent protein.
