Fig. 5. Effects of Der p2 with or without LPS on expression of mRNA encoding Toll-like receptors and cytokines in BEAS-2B cells cultured for 3, 6, and 16 hours. (A) Der p2 notably increased the expression of mRNA encoding IL-6 and IL-8, and this effect was further augmented in the presence of LPS. There was little or no change in the expression of mRNA encoding TLR4, whereas TLR2 and IL-1β were somewhat increased, especially in conjunction with LPS. Quantitative data on band densities were acquired by densitometric analysis and expressed relative to those of the β-actin loading control, which are shown under the bands. (B) Densitomeric analysis data are presented as the mean±SEM. A representative image and an actual figure representative of the 3 independent experiments with similar findings are shown.
