Skip to main content
. 2015 May 1;128(9):1835–1847. doi: 10.1242/jcs.167643

Fig. 4.

Fig. 4.

Silencing of Bmal1 promotes HIB1B brown preadipocyte terminal differentiation. (A) Immunoblot analysis of Bmal1 protein expression in HIB-1B cells with scrambled control shRNA (shSC) or stable shRNA-mediated Bmal1 knockdown (shBmal1). (B) The effect of stable Bmal1 knockdown on HIB-1B differentiation as shown by BODIPY and Mitotracker staining at day 3 of differentiation. (C) RT-qPCR analysis of brown adipocyte marker gene expression in shSC and shBmal1 cells (n = 3). Data show the mean±s.e.m. *P<0.05; **P<0.01 for shBmal1 versus shSC. (D) Immunoblot analysis of brown adipogenic markers during differentiation day 1–4 (D1–4) of shSC and shBmal1 cells, and their response to forskolin at day 4.