Fig. 1.

Cad6B localizes to the cytoplasm. (A–A″) FlpIn-wtCad6B cells were fixed and immunostained for Cad6B (green) and Na⁺K⁺ ATPase (red). Panels represent the 3D composite of several Z-stack images acquired with the confocal. Cad6B colocalizes with several cytoplasmic puncta containing Na⁺K⁺ ATPase (A″, arrowheads). (B–B″) Embryos possessing neural crest cells initiating EMT were fixed and immunostained for Cad6B (green) and Na⁺K⁺ ATPase (red). Cad6B colocalizes with Na⁺K⁺ ATPase-positive puncta (B″, arrowheads), with membrane Cad6B denoted by arrows. (C,C′) Dorsal neural folds possessing premigratory neural crest cells were explanted, allowed to undergo EMT, fixed and immunostained for Cad6B. C′ is a higher magnification view of the boxed region in C. Cad6B localizes to the cytoplasm (C′, arrowheads). Scale bars: 10 µm.