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. 2015 May 1;128(9):1773–1786. doi: 10.1242/jcs.164426

Fig. 4.

Fig. 4.

Cad6B possess a functional endocytic motif. (A) Soluble (sol) and insoluble (insol) fractions from FlpIn-wtCad6B, -LI645AA and -EED666AAA cells were subjected to SDS-PAGE. (B) Densitometric ratios of insoluble:soluble Cad6B from triplicate blots. (C) FlpIn cells expressing Cad6B constructs were surface-biotinylated and incubated at 37°C, followed by biotin immunoprecipitation (IP) with Streptavidin–agarose and SDS-PAGE. (D) Quantification of the internalized biotinylated fraction (internalized biotinylated protein/total surface biotinylated protein). (E) FlpIn cells expressing Cad6B constructs were treated with cycloheximide (CHX), lysed and subjected to SDS-PAGE. Irrelevant lanes between time 0 and the other two time points were cropped out of the LI645AA immunoblot. (F) Quantification of the densitometric ratios (Cad6B:actin) from E. Data show the mean±s.e.m.; *P<0.05 (B,D), *P<0.001 (F).