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. 2015 May 1;128(9):1773–1786. doi: 10.1242/jcs.164426

Fig. 5.

Fig. 5.

Cad6B does not colocalize with caveolin-1 but partially colocalizes with clathrin in vitro. (A–A″) FlpInwtCad6B cells were fixed and immunostained for Cad6B (green) and caveolin-1 (red). Carets show absence of Cad6B and caveolin-1 colocalization. FlpIn cells expressing wtCad6B (B–B″), LI645AA (C–C″) and EED666AAA (D–D″) were fixed, and immunostaining was performed for Cad6B (green) and clathrin (red). Panels represent single confocal plane images. Arrowheads in B″,C″,D″ show Cad6B and clathrin colocalization, and carets point to Cad6B puncta not colocalizing with clathrin. Inset boxes show the original image, with the asterisks indicating the location of the higher magnification field in the main panels. Scale bars: 10 µm.