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. 2015 May 1;128(9):1773–1786. doi: 10.1242/jcs.164426

Fig. 8.

Fig. 8.

Cad6B undergoes dynamin-dependent macropinocytosis. Vehicle (DMSO, A–A″), dynasore (B–B″), latrunculin A (C–C″) or EIPA (D–D″) was added to the explant medium. Explants were incubated to allow for EMT, fixed and immunostained for Cad6B (green) and p120-catenin (red; A′,A″,B′,B″,D′,D″) or stained with phalloidin (red; C′,C″). The arrow and arrowhead in A″ represent Cad6B and p120-catenin co-staining at the membrane and cytoplasm, respectively. Arrowheads in B″,D″ show large Cad6B and p120-catenin double-positive cytoplasmic puncta. Arrows in C″ indicate membrane-associated Cad6B. Carets in A″,B″,D″ point to Cad6B-positive, p120-catenin-negative puncta. Panels represent the 3D composite of several Z-stack images acquired with the confocal. Insets show the original image, with the asterisks in A–D indicating the location of the higher magnification field in the main panels. Scale bars: 10 µm.