Fig. 1.

MEI4 expression in pre-meiotic and preleptotene cells. Cryosections of testes from wild-type (A,B,E,F) and Mei4^−/− (C,D,G,H) adult mice were immunostained with an anti-MEI4 (red) antibody in combination with an anti-cKIT antibody (green) or in vivo EdU labeling (green). In vivo EdU labeling for 1 h allows the visualization of cells undergoing DNA replication. DNA was stained with DAPI (blue). The stage of wild-type seminiferous epithelium (VI in panels A,B; VII in panels E,F) was determined based on DAPI staining. Some non-specific cytoplasmic signal is observed with the anti-MEI4 antibody. pL, preleptotene stage; B-sg, B type spermatogonia. White arrows in F indicate spermatocytes at the pachytene stage. Scale bars: 10 µm (A,C,E,G); 5 µm (B,D,F,H).