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. 2015 May 1;128(9):1800–1811. doi: 10.1242/jcs.165464

Fig. 3.

Fig. 3.

Recruitment of MEI4 on meiotic chromosome axes is MEI1-dependent. Immunostaining with an anti-MEI4 antibody (red) and DAPI (blue) after in vivo EdU labeling (green) of cryosections of testes from 21-day-old Mei1+/− (A) and Mei1−/− (B) mice. MEI4 and SYCP3 were monitored on chromosome spreads from wild-type (C), Mei1−/− (D) and Mei4−/− (E) mutant testes. (F) The absence of MEI1 did not affect MEI4 protein levels as determined by MEI4 immunoprecipitation in protein extracts of juvenile testes (18 dpp) from wild-type and Mei1−/− mice using a crosslinked anti-MEI4 antibody. Scale bars: 10 µm.