Fig. 5.

The REC8 and RAD21L cohesin subunits are involved in MEI4 localization. The number of MEI4 foci at leptotene is lower in chromosome spreads from both Rec8^−/− and Rad21L^−/− than in wild-type mice (A–C); this effect was more pronounced in the double Rec8⁻ Rad21L^−/− mutant (D). Scale bars: 5 µm. (E) Quantification (the mean is indicated) of MEI4 foci in the different mouse strains at leptotene and zygotene stages. P-values are from two-sided Mann–Whitney tests. n, number of nuclei. The number of DMC1 foci detected and quantified in wild-type and mutant mice in a previous study (Ishiguro et al., 2014) is plotted on the right y-axis.