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. 2015 Apr 30;7(5):3166–3183. doi: 10.3390/nu7053166

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effect of α-naphthoflavone (α-NF) on lipid accumulation in differentiated adipocytes. Cells were treated with various concentrations of α-NF for 16 h. Intracellular lipid accumulation was observed by oil-red-O staining (100× magnification) (A) and quantified (B) by the method described in “Materials and Methods”. Triglyceride (TG) contents (C) were quantified with a commercial kit. Pictures are representative of three independent experiments. Values are the mean ± SD from three measurements. Data with different letters significantly differ (p < 0.05).

Effect of α-naphthoflavone (α-NF) on lipid accumulation in differentiated adipocytes. Cells were treated with various concentrations of α-NF for 16 h. Intracellular lipid accumulation was observed by oil-red-O staining (100× magnification) (A) and quantified (B) by the method described in “Materials and Methods”. Triglyceride (TG) contents (C) were quantified with a commercial kit. Pictures are representative of three independent experiments. Values are the mean ± SD from three measurements. Data with different letters significantly differ (p < 0.05).