Figure 3.

Effects of α-naphthoflavone (α-NF) on vascular tube formation in endothelial cells activated with α-NF-treated conditioned medium (CM). After full differentiation, adipocytes were incubated with Dulbecco’s modified Eagle’s medium (DMEM) containing 1% fetal bovine serum (FBS) in the presence of various concentrations of α-NF for 24 h, and CM was then used to cultivate endothelial EA hy926 cells, which were grown on Matrigel-coated plates for another 24 h. Formation of tube-like structures was observed and photographed under a phase-contrast microscope after staining with acetomethoxy derivate of calcein (calcein–AM) fluorescent dye (100× magnification). Pictures are representative of three independent experiments.