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. 2015 Apr 30;7(5):3166–3183. doi: 10.3390/nu7053166

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effect of α-naphthoflavone (α-NF) on vascular endothelial growth factor (VEGF) (A), interleukin (IL)-6 (B), nitric oxide (NO) (C), and insulin growth factor (IGF)-1 (D) secretion in culture medium from differentiated adipocytes. After full differentiation, adipocytes were treated with various concentrations of α-NF in Dulbecco’s modified Eagle’s medium (DMEM) containing 1% fetal bovine serum (FBS) for 16 h. The medium was collected to analyze nitrite using the Griess reagent, IGF-1, VEGF, and IL-6 by ELISA kits. Values are the mean ± SD from three measurements, and data with different letters significantly differ (p < 0.05).

Effect of α-naphthoflavone (α-NF) on vascular endothelial growth factor (VEGF) (A), interleukin (IL)-6 (B), nitric oxide (NO) (C), and insulin growth factor (IGF)-1 (D) secretion in culture medium from differentiated adipocytes. After full differentiation, adipocytes were treated with various concentrations of α-NF in Dulbecco’s modified Eagle’s medium (DMEM) containing 1% fetal bovine serum (FBS) for 16 h. The medium was collected to analyze nitrite using the Griess reagent, IGF-1, VEGF, and IL-6 by ELISA kits. Values are the mean ± SD from three measurements, and data with different letters significantly differ (p < 0.05).