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. 2015 Apr 30;7(5):3166–3183. doi: 10.3390/nu7053166

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effects of α-naphthoflavone (α-NF) on the aryl hydrocarbon receptor (AhR), the AhR nuclear translocator (ARNT), cytochrome P4501B1 (CYP1B1), estrogen receptor (ER), hormone-sensitive lipase (HSL), glycerol phosphate dehydrogenase (GPDH), nuclear factor erythroid 2-related factor 2 (NRF2), and vascular endothelial growth factor receptor (VEGFR) protein expressions by differentiated adipocytes. After full differentiation, adipocytes were treated with various concentrations of α-NF in Dulbecco’s modified Eagle’s medium (DMEM) containing 1% fetal bovine serum (FBS) for 16 h, and proteins were collected and then measured with a Western blot analysis. Levels of protein are expressed relative to the cells treated with no α-NF after being normalized by β-actin expression. Pictures are representatives of three independent experiments.