Table 1.
Composition of experimental diets (as formulated).
| Macronutrient | Diet “L” (0.4 mg Folate + 0.08 mg Se/kg) | Diet “H” (2.4 mg Folate+ 0.44 mg Se/kg) | |||
|---|---|---|---|---|---|
| gm % | kcal% | gm % | kcal% | ||
| Protein | 20 | 17 | 20 | 17 | |
| Carbohydrate | 49 | 42 | 50 | 43 | |
| Fat | 21 | 40 | 21 | 40 | |
| Total | 99 | 100 | |||
| kcal/g | 4.68 | 4.68 | |||
| Ingredient | gm | kcal | gm | kcal | |
| Casein | 195 | 780 | 195 | 780 | |
| dl-methionine | 3 | 12 | 3 | 12 | |
| Corn Starch | 50 | 200 | 50 | 200 | |
| Matodextrin 10 | 100 | 400 | 100 | 400 | |
| Sucrose | 341 | 1364 | 341 | 1364 | |
| Cellulose BW200 | 50 | 0 | 50 | 0 | |
| Milk Fat Anhydrous | 200 | 1800 | 200 | 1800 | |
| Corn Oil | 10 | 90 | 10 | 90 | |
| Mineral Mix S10001 † | 0 | 35 | |||
| Sodium Selenite (45.7% Se) | 0.000067 | 0.00058 | |||
| Mineral mix S19101 (no Se) | 35 | 0 | |||
| Calcium Carbonate | 4 | 4 | |||
| Vitamin Mix V10001 ‡ | 0 | 10 | 40 | ||
| Vitamin mix V14901 (no Fo) | 10 | 40 | 0 | ||
| Folic Acid | 0.00032 | 0.00032 | |||
| Choline Bitartrate | 2 | 2 | |||
| Cholesterol USP | 1.5 | 1.5 | |||
| Ethoxyquin | 0.04 | 0.04 | |||
| FD&C Yellow Dye #5 | 0.05 | 0 | |||
| FD&C Red Dye #40 | 0 | 0.05 | |||
| Total | 1001.59 | 4686 | 1001.59 | 4686 | |
† S1001: Calcium Phosphate, Dibasic, 29.5% Ca, 22.8% P 500 gm; Magnesium Oxide, 60.3% Mg 24 gm; Potassium Citrate, 1 H2O, 36.2% K 220 mg; Potassium Sulfate, 44.9% K, 18.4% S 52 gm; Sodium Chloride, 39.3% Na, 60.7% Cl 74 gm; Chromium K Sulfate, 12 H2O, 10.4% Cr 0.55 gm; Cupric Carbonate, 57.5% Cu 0.3 gm; Potassium Iodate, 59.3% I 0.01 gm; Ferric Citrate, 21.2% Fe 6 gm; Manganous Carbonate, 47.8% Mn 3.5 gm; Sodium Selenite, 45.7% Se 0.01 gm (not present in S19101); Zinc Carbonate, 52.1% Zn 1.6 gm; Sucrose 118.03 gm. ‡ V10001: Vitamin A Palmitate, 500,000 IU/gm 0.8 gm; Vitamin D3, 100,000 IU/gm 1 gm; Vitamin E Acetate, 500 IU/gm 10 gm; Menadione Sodium Bisulfite, 62.5% menadione 0.8 gm; Biotin, 1.0% 2 gm; Cyancocobalamin, 0.1% 1 gm; Folic Acid 0.2 gm; Nicotinic Acid 3 gm; Calcium Pantothenate 1.6 gm; Pyridoxine-HCl 0.7 gm; Riboflavin 0.6 gm; Thiamin HCl 0.6 gm; Sucrose 978.42 gm.