Fig. 2.

Curcumin and bicalutamide increased phosphorylation of ERK1/2 and SAPK/JNK. a-b PC3 and DU145 cells were treated with curcumin (40 μM) in the indicated times, and cell lysate was harvested and the expression of the phosphorylated or total protein of ERK1/2 and SAPK/JNK were measured by Western blot analysis using corresponding antibodies. GAPDH was used as loading control. c-d PC3 and DU145 cells were treated with curcumin (40 μM) and bicalutamide (30 μM) for 8 h. Afterwards, the phosphorylation and expression of ERK1/2 and SAPK/JNK were detected by Western blot. Values in bar graphs were given as the mean ± SD from three independent experiments performed in triplicate. *indicates significant difference as compared to the untreated control group (P < 0.05). **Indicates significant difference from curcumin treated alone (P < 0.05)