Figure 2.

Accelerating effect of MIOX on the altered mitochondrial dynamics, associated proteins, and mitochondrial membrane potential under HG ambience. (A, a and b and C) MIOX overexpressing LLC-PK1 cells had higher percentages of fragmented mitochondria under LG or HG ambience. (A, c and d) Under LG ambience, MIOX-transfected cells had Drp1 and Mfn2 fluorescence intensity similar to the control cells treated with HG. (B) A dose-dependent increase in the expression of Drp1 and Fis1 was seen under HG ambience, whereas the expression of Mfn2 was slightly reduced. Translocation of Drp1 to mitochondria was also seen in a dose-dependent manner, and it was accentuated in MIOX overexpressing cells. (A, e and D) This was associated with a decrease in TMRE staining, indicating loss of mitochondrial voltage potential (ΔΨm). Con, control.