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. 2015 May 28;5:10144. doi: 10.1038/srep10144

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Copyright © 2015, Macmillan Publishers Limited

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SERS barcoding and screening of solid-phase peptides library. a) Model penta-peptides containing HPQ and biotin-loaded TentaGel (TG) beads and their corresponding SERS barcodes using SERS IDs combinations. b) Schematic diagram of lead peptides with high binding affinity against streptavidin-loaded TG beads and the identification process based on a fluorescence signal for binding affinity quantification and SERS barcodes for peptide sequencing. c) Optical image of TG bead mixtures after the streptavidin-binding assay, and SERS barcodes from the TG beads. d) Identification of bio-ligands through decoding of their SERS barcodes. e) Histogram for fluorescence signal variation of streptavidin-coated fluorescent nanoparticles binding to TG beads.