Fig. 5.

DNA synthesis in human dermal fibroblasts treated with siRNAs. Following siRNA trasfection and synchronization, cells were labeled with BrdU for 24 h. Then, the cells were fixed, and incubated with anti-BrdU antibodies. BrdU incorporation was quantified by a colorimetric reaction (absorbance at 460 nm). Presented results are mean values from at least three independent experiments, with error bars indicating SD. In each experiment, DNA synthesis level measured in untreated cells was used as a control value (100 %, dashed line). Statistically significant differences relative to the control are indicated by asterisks