Table 1.
Relative expression levels of porcine and bacterial genes
| Gene | Gene product | Control 3 | 6 h p.i. 3 | 12 h p.i. 3 | 24 h p.i. 3 | 48 h p.i. 3 | P value 4 |
|---|---|---|---|---|---|---|---|
| Porcine 1 – Genes involved in pattern recognition | |||||||
| TLR4 | Toll-like receptor 4 | 1.0 ± 0.1 | 12.6 ± 1.6 | 8.3 ± 2.6 | 4.1 ± 1.2 | 3.7 ± 0.6 | 9.00E-08 |
| CD14 | Cluster of differentiation 14 | 1.0 ± 0.2 | 8.7 ± 0.8 | 7.6 ± 2.1 | 4.5 ± 1.1 | 4.1 ± 0.4 | 8.00E-08 |
| MD2 | Myeloid differentiation protein-2 | 1.0 ± 0.1 | 3.4 ± 0.4 | 3.2 ± 0.4 | 2.0 ± 0.3 | 2.0 ± 0.4 | 1.06E-05 |
| LBP | Lipopolysaccharide-binding protein | 1.0 ± 0.4 | 8.8 ± 1.9 | 8.6 ± 4.4 | 8.6 ± 2.0 | 24.7 ± 4.5 | 3.38E-05 |
| MYD88 | Myeloid differentiation primary response 88 | 1.0 ± 0.1 | 5.2 ± 0.7 | 4.6 ± 1.5 | 1.8 ± 0.3 | 2.5 ± 0.6 | 7.25E-06 |
| IRF3 | Interferon regulatory factor 3 | 1.0 ± 0.1 | 1.4 ± 0.2 | 1.7 ± 0.4 | 1.0 ± 0.1 | 1.0 ± 0.1 | 0.021 |
| SFTPA | Surfactant protein A | 1.0 ± 0.1 | 0.6 ± 0.1 | 0.7 ± 0.2 | 0.7 ± 0.2 | 0.3 ± 0.2 | 0.014 |
| SFTPD | Surfactant protein D | 1.0 ± 0.1 | 0.6 ± 0.1 | 1.0 ± 0.4 | 0.6 ± 0.1 | 0.5 ± 0.3 | NS |
| Porcine 1 – Genes involved in involved in iron metabolism | |||||||
| CD163 | CD163 molecule | 1.0 ± 0.1 | 0.8 ± 0.1 | 3.0 ± 1.1 | 2.1 ± 0.2 | 3.0 ± 0.7 | 0.022 |
| HP | Haptoglobin | 1.0 ± 0.2 | 2.2 ± 0.1 | 2.1 ± 0.2 | 1.2 ± 0.2 | 1.2 ± 0.5 | 0.004 |
| TF | Transferrin | 1.0 ± 0.1 | 0.4 ± 0.1 | 0.5 ± 0.2 | 0.3 ± 0.1 | 0.1 ± 0.0 | 1.31E-04 |
| LTF | Lactotransferrin | 1.0 ± 0.6 | 10.2 ± 5.9 | 30.4 ± 22.0 | 10.5 ± 6.0 | 7.1 ± 3.5 | NS |
| Porcine 1 – Cytokines involved in innate immune modulation and inflammation | |||||||
| IL1B | Interleukin-1 β | 1.0 ± 0.2 | 414 ± 50 | 253 ± 93 | 175 ± 41 | 209 ± 27 | 7.20E-07 |
| IL1RN | Interleukin-1 receptor antagonist | 1.0 ± 0.2 | 80.1 ± 12.9 | 53.4 ± 21.2 | 40.7 ± 9.7 | 37.8 ± 3.6 | 7.50E-07 |
| IL6 | Interleukin-6 | 1.0 ± 0.1 | 1024 ± 67 | 256 ± 99 | 80.2 ± 29.1 | 48.9 ± 22.3 | 6.00E-08 |
| IL8 | Interleukin-8 | 1.0 ± 0.2 | 261 ± 19 | 181 ± 69 | 91.0 ± 26.1 | 104 ± 19 | 1.28E-06 |
| IL17A | Interleukin-17A | 1.0 ± 0.3 | 325 ± 54 | 116 ± 55 | 46.1 ± 13.4 | 21.5 ± 4.1 | 1.49E-05 |
| IL18 | Interleukin-18 | 1.0 ± 0.1 | 3.7 ± 0.4 | 7.1 ± 2.8 | 6.5 ± 2.1 | 6.0 ± 0.8 | 1.51E-04 |
| IFNG | Interferon-gamma | 1.0 ± 0.2 | 3.1 ± 0.4 | 1.1 ± 0.3 | 1.0 ± 0.2 | 0.3 ± 0.1 | 1.19E-05 |
| TNF | Tumor necrosis factor | 1.0 ± 0.1 | 4.8 ± 0.6 | 2.5 ± 0.5 | 2.0 ± 0.7 | 1.8 ± 0.3 | 3.20E-04 |
| CSF2 | GM-CSF | 1.0 ± 0.1 | 4.4 ± 0.6 | 2.6 ± 0.9 | 1.4 ± 0.3 | 0.6 ± 0.3 | 4.69E-05 |
| Porcine 1 – Genes involved in the complement system and other functions | |||||||
| C3 | Complement component 3 | 1.0 ± 0.1 | 1.4 ± 0.1 | 1.0 ± 0.1 | 0.9 ± 0.1 | 0.7 ± 0.2 | 0.015 |
| CFB | Complement factor B | 1.0 ± 0.1 | 5.6 ± 0.4 | 3.4 ± 0.9 | 2.7 ± 0.4 | 2.9 ± 1.1 | 1.28E-05 |
| CFD | Complement factor D | 1.0 ± 0.1 | 1.4 ± 0.2 | 1.1 ± 0.1 | 1.1 ± 0.1 | 0.8 ± 0.1 | NS |
| MASP2 | Mannan-binding lectin serine peptidase 2 | 1.0 ± 0.1 | 0.4 ± 0.0 | 0.5 ± 0.2 | 0.3 ± 0.1 | 0.2 ± 0.0 | 1.14E-04 |
| SAA | Serum amyloid A | 1.0 ± 0.2 | 15.3 ± 2.3 | 20.4 ± 11.0 | 68.9 ± 19.6 | 110.2 ± 19.6 | 7.57E-06 |
| TNFAIP3 | Tumor necrosis factor, alpha-induced protein 3 | 1.0 ± 0.1 | 17.5 ± 1.7 | 7.7 ± 2.6 | 4.1 ± 1.0 | 3.7 ± 0.4 | 8.00E-07 |
| GZMB | Granzyme B | 1.0 ± 0.5 | 3.7 ± 1.6 | 1.0 ± 0.5 | 1.3 ± 0.3 | 0.4 ± 0.1 | 0.0069 |
| Bacterial 2 – Genes involved in cell wall membrane biogenesis | |||||||
| kdsB | 3-deoxy-manno-octulosonate cytidylyltransferase | NA | 2.6 ± 0.5 | 1.7 ± 0.2 | 1.2 ± 0.1 | 1.0 ± 0.1 | 7.97E-04 |
| wzxE | Lipopolysaccharide biosynthesis protein | NA | 1.7 ± 0.1 | 1.4 ± 0.1 | 1.2 ± 0.1 | 1.0 ± 0.1 | 1.50E-03 |
| ompA | Outer membrane protein P5 | NA | 1.0 ± 0.1 | 2.5 ± 0.3 | 3.6 ± 0.5 | 3.6 ± 0.7 | 3.11E-05 |
| mltC | Murein transglycosylase C | NA | 1.9 ± 0.2 | 1.7 ± 0.1 | 1.3 ± 0.1 | 1.0 ± 0.1 | 8.20E-05 |
| palA | Outer membrane protein | NA | 1.7 ± 0.1 | 1.4 ± 0.2 | 1.0 ± 0.1 | 1.1 ± 0.1 | 9.01E-04 |
| tolA | Cell envelope integrity inner membrane protein | NA | 1.0 ± 0.1 | 1.5 ± 0.1 | 1.5 ± 0.2 | 1.6 ± 0.1 | 9.99E-03 |
| wecC | UDP-N-acetyl-D-mannosamine dehydrogenase | NA | 2.2 ± 0.1 | 1.8 ± 0.2 | 1.5 ± 0.1 | 1.0 ± 0.2 | 2.72E-04 |
| ompP4 5 | Lipoprotein E | NA | 4.2 ± 0.6 | 2.8 ± 0.7 | 1.4 ± 0.3 | 1.0 ± 0.2 | 4.07E-04 |
| Bacterial 2 – Genes involved in iron uptake | |||||||
| afuB | Ferric transport system permease protein fbpB | NA | 3.9 ± 0.4 | 2.6 ± 0.3 | 1.6 ± 0.2 | 1.0 ± 0.1 | 2.33E-06 |
| hgbA | Hemoglobin-binding protein | NA | 1.3 ± 0.1 | 1.0 ± 0.1 | 1.0 ± 0.2 | 1.0 ± 0.2 | NS |
| Bacterial 2 – Gene involved in exotoxin production | |||||||
| apxIIA | RTX-II toxin determinant A | NA | 7.0 ± 0.7 | 3.0 ± 0.7 | 2.3 ± 0.4 | 1.0 ± 0.3 | 5.16E-06 |
| Bacterial 2 – Genes involved in adhesion and competence | |||||||
| comEA | Fibronectin adhesion protein | NA | 5.8 ± 0.8 | 2.8 ± 0.5 | 1.5 ± 0.4 | 1.0 ± 0.2 | 5.33E-05 |
| csgG | Putative lipoprotein | NA | 1.2 ± 0.1 | 1.2 ± 0.2 | 1.0 ± 0.2 | 1.0 ± 0.2 | NS |
| Bacterial 2 – Genes other functions | |||||||
| dnaK | Chaperone protein Dna | NA | 2.9 ± 0.4 | 2.6 ± 1.3 | 2.0 ± 0.8 | 1.0 ± 0.5 | NS |
| mgsA | Methylglyoxal synthase | NA | 1.8 ± 0.2 | 1.3 ± 0.2 | 1.0 ± 0.1 | 1.4 ± 0.2 | 0.027 |
1Sample sizes: Control: n = 7; 6 h p.i.: n = 6; 12 h p.i.: n = 5; 24 h p.i.: n = 8; 48 h p.i.: n = 5.
2Number of data points: 6 h p.i.: n = 6; 12 h p.i.: n = 4; 24 h p.i.: n = 7; 48 h p.i.: n = 5.
3Expression is given ± SEM.
4P values are included for genes that were differentially expressed during the course of the study (ANOVA, P < 0.05). NS = not significant.
5Gene also involved in iron uptake (heme acquisition).