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Efficiency of PVX- and CPMV-based vectors. Coomassie brilliant blue stained gel (a) and western blot (b) of proteins isolated form N. benthamiana plants and separated by SDS-PAGE. M, − molecular weight marker (kD); 1 and 2, − total soluble proteins isolated at fourth day after agroinfiltration from leaves infiltrated with vector pA7248AMV-Flg-4M and pEAQ-Flg-4M, respectively; 3, − total soluble proteins isolated from empty leaves. Antibodies against M2e were used in western blotting
