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. 2015 May 29;15:42. doi: 10.1186/s12896-015-0164-6

Fig. 3.

Fig. 3

Expression and purification of Flg-4M protein. Coomassie brilliant blue stained gel (a) and western blot (b) of proteins isolated form N. benthamiana plants and separated by SDS-PAGE. M, − molecular weight marker (kD); 1, − total soluble proteins isolated from empty leaves; 2, − total soluble proteins isolated at fourth day after agroinfiltration from leaves infiltrated with vector pA7248AMV-Flg-4M; 3, − purified Flg-4M protein. Antibodies against M2e were used in western blotting