Figure 3.
PTP-1B antisense inhibited the effects of Ang II on insulin signaling. PTP-1B expression was attenuated by PTP-1B antisense after 6 hours of transfection (A). Ang II failed to inhibit tyrosine phosphorylation of IRS-1(B) and activity of Akt - induced by insulin after VSMC was transfected with PTP-1B antisense (C) * p<0.05 vs. Ang II+insulin, Ang II+insulin+PTP-1B sense. PTP-1B antisense blocked the combined effect of insulin and Ang II on DNA synthesis (D) #p<0.05 vs. control group without Ang II, *p<0.05 vs. Ang II alone, &p<0.05 vs. Ang II+insulin+PTP-1B antisense. Representative blots are shown and the results represent the mean ± SEM of 5 independent experiments.