Figure 3.

Serotype cross reactivity of sera and bulk culture antibody profile in BLT NSG mice. (a) Inactivated DENV lysates from all four serotypes of dengue were immobilized on ELISA plates. A 1:10 dilution of sera from immune or naïve BLT NSG mice was tested for cross-reactivity to DENV lysates. Splenocytes from mice infected 7 or 45 days prior with 10⁸ PFU DENV-2 S16803 by the s.c. route were stimulated with CpG + IL-2 and EBV. Culture supernatants obtained 14 days later were used as a source of antibodies to perform an ELISA. (b) IgM responses to DENV-2 antigen and DENV-2 rE protein were tested using undiluted bulk supernatants from splenocytes of acutely infected (open squares) and immune (open circles) mice and a 1:10 dilution of sera