Figure 6.

Neutralization activity and antibody avidity and of hAbs isolated from DENV-infected BLT-NSG mice. Neutralization activity of representative hAbs isolated at (a) d7 or (b) d45 using a flow-based neutralization assay. Percent neutralization of serially diluted supernatants from stimulated B cells that secrete hAbs was calculated. Avidity indices of hAbs to (c) DENV-2 inactivated lysates and (d) Con A-immobilized DENV-2. The avidity index was calculated as the ratio of the O.D. with urea to the O.D. without urea multiplied by 100. P values, as determined by Mann–Whitney U test, are shown for statistically significant comparisons. (e) RNA was isolated from three of the B cell lines, and the antibody mRNA was converted to cDNA and sequenced